Quantitative electrochemical detection of the heart failure marker NT-proBNP using a simple paper-based sensor
This presentation describes a new, low-cost, appropriately sensitive paper universal diagnostic device for electrochemical detection of analytes ranging from biological weapons to DNA to the heart failure biomarker NT-proBNP. To satisfy this objective, we initiated development of a quantitative sensor using a magnetic microbead supported silver nanoparticle metalloimmunoassay. The sensor integrates picomolar affinity antibodies (or DNA) with an easily handled, but sophisticated, electrochemical detection platform: the NoSlip. This device exhibits quantitative detection of analytes present at sub-picomolar levels using non-enzymatic signal amplification. Total assay time is ~5 min and matrices at this stage of development include urine and buffer. The cost is ~0.40 USD/sensor (not including reagents).
Richard M. Crooks received B.S. and doctoral degrees in chemistry from the University of Illinois and The University of Texas at Austin. His independent career has been split between Texas A&M University and UT-Austin, where he is presently holds the Welch Chair in Materials Chemistry. His research program focuses on biosensing and electrocatalysis.